| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.197043 |
| Chromosome: | chromosome 14 |
| Location: | 2605416 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g625901 | (1 of 3) PF01985 - CRS1 / YhbY (CRM) domain (CRS1_YhbY) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCCAGGAAAGCGGGATGGCCCGGCACTG |
| Internal bar code: | TCGGAGCTTTCGGGTTTGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 761 |
| LEAP-Seq percent confirming: | 99.6705 |
| LEAP-Seq n confirming: | 1210 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACAGAGACTCCTGCGGAC |
| Suggested primer 2: | TTCTGTTGCTGTTTGACGCT |