| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.197061 |
| Chromosome: | chromosome 6 |
| Location: | 7561656 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g300850 | (1 of 1) PTHR22715//PTHR22715:SF0 - TRANSFORMING GROWTH FACTOR BETA REGULATED GENE 1 // TRANSFORMING GROWTH FACTOR BETA REGULATOR 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTCCGTTCGCGGCTTTGGGACTTGCGGC |
| Internal bar code: | CTAAGAGGTATTGTCGCTCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 33 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCAACACCCTCGTCCTC |
| Suggested primer 2: | TATACGGTAGCATAGCGGGG |