| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.197065 |
| Chromosome: | chromosome 7 |
| Location: | 3667660 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337350 | GT90F8,GT90-8 | GT90 family protein 8; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATGTACTCATGGTATGCACTGCAGCTCC |
| Internal bar code: | GCTTTCTTTAGCCATTTTATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 300 |
| LEAP-Seq percent confirming: | 85.9813 |
| LEAP-Seq n confirming: | 1104 |
| LEAP-Seq n nonconfirming: | 180 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTCTTCTTCTGCCTTCGC |
| Suggested primer 2: | CAAGACCGTATGGCCAAGTT |