| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.197082 |
| Chromosome: | chromosome 4 |
| Location: | 1561491 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213500 | TMG8 | (1 of 1) PTHR23417//PTHR23417:SF18 - 3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE // SUBFAMILY NOT NAMED; tRNA (guanine-N7)-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCTCTGCCGCGCTGGGCTCAGGTGGGCG |
| Internal bar code: | GGAGGTTCAAGGGCTTTAATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 691 |
| LEAP-Seq percent confirming: | 98.4165 |
| LEAP-Seq n confirming: | 3294 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTAGCAAGCCCAACATGG |
| Suggested primer 2: | ACTTTGTACGGCATCGTTCC |