| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.197115 |
| Chromosome: | chromosome 4 |
| Location: | 1654310 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213050 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGATGTCGCTGGCGACTCGCGTCAGCA |
| Internal bar code: | CAAATTCAGTGCGGGGGTGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 305 |
| LEAP-Seq percent confirming: | 97.6546 |
| LEAP-Seq n confirming: | 1374 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTATATGTGGGCGGGAAC |
| Suggested primer 2: | GCATTGGTGGAGGAGTGTTT |