Insertion junction: LMJ.RY0402.197143_4

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.197143
Chromosome:chromosome 12
Location:7801150
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g553500 ANK15 Predicted protein with ankyrin repeats; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGGTGCGCGGGTTTTGAACAGTCGGCAC
Internal bar code:GTCGTTTAGACTCCAGTGGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1042
LEAP-Seq percent confirming:99.0698
LEAP-Seq n confirming:1491
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCACACGGCATTCTACTC
Suggested primer 2:AGGTATTGGGCACATGGTGT