Insertion junction: LMJ.RY0402.197164_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.197164
Chromosome:chromosome 8
Location:239486
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre08.g358552 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTGTACCTGGCTACACCTTCACTTCTTC
Internal bar code:GCGTTCGGGCGGGCCGGTCGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:117
LEAP-Seq percent confirming:50.3216
LEAP-Seq n confirming:2738
LEAP-Seq n nonconfirming:2703
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCAGCAGCAAAAACAACA
Suggested primer 2:CCCACACACACACAGACACA