| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.197212 |
| Chromosome: | chromosome 15 |
| Location: | 1026595 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g638750 | CLR3,RAP17,CLR20,OPR83 | RAP-domain containing protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAACGGTCGACAACGTCGACCACTAGCA |
| Internal bar code: | AGCTTGTGGGTCGGGTCAGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 753 |
| LEAP-Seq percent confirming: | 98.7104 |
| LEAP-Seq n confirming: | 842 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGAAGCTGAGGCATTCAA |
| Suggested primer 2: | ATGCGACTCCTCTGCTTGTT |