| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.197232 |
| Chromosome: | chromosome 12 |
| Location: | 6470081 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538250 | (1 of 8) 2.4.1.5 - Dextransucrase / Sucrose 6-glucosyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCAGGCTCCGCGCCGGCCCTACACCAG |
| Internal bar code: | CGCGTGAAAGCGGGTGCAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 459 |
| LEAP-Seq percent confirming: | 99.6154 |
| LEAP-Seq n confirming: | 518 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGTGGCTTTCTACAGCTT |
| Suggested primer 2: | GCAACACTGACAGCACCAGT |