| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.197276 |
| Chromosome: | chromosome 6 |
| Location: | 7759653 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g302200 | EFH3 | (1 of 1) PF13202//PF14252 - EF hand (EF-hand_5) // Domain of unknown function (DUF4347) (DUF4347); EF-hand Calcium binding protein with DUF4346 domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGCGATAAGGTGTGAGCGGCGGCTATAG |
| Internal bar code: | CCGGTGACGGCGCATCGACGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 607 |
| LEAP-Seq percent confirming: | 88.1115 |
| LEAP-Seq n confirming: | 1549 |
| LEAP-Seq n nonconfirming: | 209 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGCTACACGCTAACGGA |
| Suggested primer 2: | TGCGCTACATCAAGAAGGTG |