Insertion junction: LMJ.RY0402.197304_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.197304
Chromosome:chromosome 1
Location:3643315
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g023550 FAP234,TPG2 Flagellar Associated Protein 234; (1 of 1) PTHR24365//PTHR24365:SF301 - TOLL-LIKE RECEPTOR // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCACAGGCAGCCGCGCACAGCCTTGCAAGC
Internal bar code:GGGAGGCCGGGAGCGCGCGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:286
LEAP-Seq percent confirming:82.7254
LEAP-Seq n confirming:1633
LEAP-Seq n nonconfirming:341
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CTCAGCTTCTGCGGTAGCAC
Suggested primer 2:AAATGATGAGGTTTGCGAGG