Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.197307 |
Chromosome: | chromosome_16 |
Location: | 6897821 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g676533 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCAGCCGCAGGTAGGTGCTAGGTGCCATG |
Internal bar code: | ACTGCACCCCCATACTTCGAGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 310 |
LEAP-Seq percent confirming: | 98.9691 |
LEAP-Seq n confirming: | 1632 |
LEAP-Seq n nonconfirming: | 17 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGGTGGTGATGCTGGTTCT |
Suggested primer 2: | CCGCCACTTTACTTCACCAT |