| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.197307 |
| Chromosome: | chromosome 16 |
| Location: | 6897821 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676533 | (1 of 1) IPR000742//IPR001599 - EGF-like domain // Alpha-2-macroglobulin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTCATGATGGCGGCGGTACGTGGGAGC |
| Internal bar code: | AGATGACTTTTTCGACGTAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1145 |
| LEAP-Seq percent confirming: | 98.5185 |
| LEAP-Seq n confirming: | 133 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTGGTGATGCTGGTTCT |
| Suggested primer 2: | CCGCCACTTTACTTCACCAT |