Insertion junction: LMJ.RY0402.197318_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.197318
Chromosome:chromosome 6
Location:8119583
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g304876 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGACTTGCTACGCGAGCTCCAACGGGCGT
Internal bar code:ACGGTCGGAGACTGTTGCGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:987
LEAP-Seq percent confirming:99.6145
LEAP-Seq n confirming:3876
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:AACCGCAATCACAATGACAA
Suggested primer 2:CTGTCGTTGTGAGGCACACT