| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.197337 |
| Chromosome: | chromosome 16 |
| Location: | 5657044 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679350 | (1 of 3) IPR003131//IPR011333 - Potassium channel tetramerisation-type BTB domain // POZ domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAAGCTCGCCCTCGCCACCACCACCCGG |
| Internal bar code: | CTGGACATAACCTTGCGTCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 336 |
| LEAP-Seq percent confirming: | 99.6187 |
| LEAP-Seq n confirming: | 12019 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACACGTCCCTACACCCACA |
| Suggested primer 2: | GTATTTCACATGCACGGCAC |