| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.197381 |
| Chromosome: | chromosome_9 |
| Location: | 291405 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g392867 | FMG1-B | Flagella membrane glycoprotein, major form | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGTAGAAGTGGTAACGTTTGATAGCGTAG |
| Internal bar code: | CCGGGGGATTGCGACTGATTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 897 |
| LEAP-Seq percent confirming: | 94.3878 |
| LEAP-Seq n confirming: | 740 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAATGTGTCCGTGACCAG |
| Suggested primer 2: | GCAACAACATTCACCACGTC |