| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.197399 |
| Chromosome: | chromosome 3 |
| Location: | 6733020 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197850 | (1 of 2) PF11267 - Domain of unknown function (DUF3067) (DUF3067) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGGCTCTAGGTCGAAGCGGATGGTGAT |
| Internal bar code: | GGTGGCAATTAAGCAACAAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1076 |
| LEAP-Seq percent confirming: | 99.941 |
| LEAP-Seq n confirming: | 1693 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGTAACCATTTGGTGCG |
| Suggested primer 2: | CATGTGCGTTTTACTGGTGG |