| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.197404 |
| Chromosome: | chromosome 13 |
| Location: | 5127371 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607500 | KU70 | ATP-dependent DNA helicase 2 subunit KU70; (1 of 1) K10884 - ATP-dependent DNA helicase 2 subunit 1 (XRCC6, KU70, G22P1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGGCGATGTAGGGGCCTAGGGGCAGCG |
| Internal bar code: | GTAAGTTTGGTTCCATCAGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1156 |
| LEAP-Seq percent confirming: | 99.4041 |
| LEAP-Seq n confirming: | 1668 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTAATGTGGCTGGCCGTAG |
| Suggested primer 2: | CCCTGATGCTTGAAACACCT |