| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.197405 |
| Chromosome: | chromosome 7 |
| Location: | 2677497 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330800 | OPR30 | OctotricoPeptide Repeat protein 30 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTATGACTGGCCCCCAACACCTGACCTCA |
| Internal bar code: | ACATCGATGCGCACCCGGCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 281 |
| LEAP-Seq percent confirming: | 96.3441 |
| LEAP-Seq n confirming: | 1344 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAAGTTGGAGGTGAAAGG |
| Suggested primer 2: | GACGTGGACTTGGTTGAGGT |