| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.197420 |
| Chromosome: | chromosome 6 |
| Location: | 5328984 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g283250 | POB9 | Proteome of basal body 9 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCTTATCGTGTACCGCCGCCAGCGTGTG |
| Internal bar code: | CCCCTGGGGACTGGCCGGGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 274 |
| LEAP-Seq percent confirming: | 92.9164 |
| LEAP-Seq n confirming: | 13327 |
| LEAP-Seq n nonconfirming: | 1016 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGACTTCTTCTGTTTTCG |
| Suggested primer 2: | TACCTACCGAATACCGAGCG |