| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.197421 |
| Chromosome: | chromosome 12 |
| Location: | 1439234 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487850 | CGL49,ARL11 | (1 of 1) K07977 - Arf/Sar family, other (ARF); ARF/SAR superfamily small monomeric GTP binding protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACTGACAAGAGGTGACCCCGCTGGTCAT |
| Internal bar code: | AATAGACATGTCGAGCCTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 898 |
| LEAP-Seq percent confirming: | 99.6947 |
| LEAP-Seq n confirming: | 5225 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGGGGCTTCGTATTTGTA |
| Suggested primer 2: | GCCCTCGGGCTTATAACTTC |