| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.197422 |
| Chromosome: | chromosome 3 |
| Location: | 5613748 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g187000 | (1 of 1) PF12325//PF12329 - TATA element modulatory factor 1 TATA binding (TMF_TATA_bd) // TATA element modulatory factor 1 DNA binding (TMF_DNA_bd) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGTCAGGCCTCGCAGCTGTCCATCGTAG |
| Internal bar code: | AAGAAGGGTTTCCCCGACCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 203 |
| LEAP-Seq percent confirming: | 99.5777 |
| LEAP-Seq n confirming: | 1179 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGAGCTTGTTGTGGTGA |
| Suggested primer 2: | TCGAGTTTATGTGCCAGCAG |