| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.197482 |
| Chromosome: | chromosome 7 |
| Location: | 1864887 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325737 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCGGTGGGGGACCAGGGGGCAGGTAGCG |
| Internal bar code: | GATGTTGCTCGGTACACGGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 557 |
| LEAP-Seq percent confirming: | 93.7238 |
| LEAP-Seq n confirming: | 4913 |
| LEAP-Seq n nonconfirming: | 329 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGCGGCAGATAGTAGTAG |
| Suggested primer 2: | CCGTTCCTCTCATCACCATT |