| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.197489 |
| Chromosome: | chromosome 6 |
| Location: | 2402580 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g268150 | CNK3 | NimA-related protein kinase; (1 of 4) PTHR24361//PTHR24361:SF411 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGACGCCATTCTGAAGAGCGACTTTGTGC |
| Internal bar code: | AGAGGCTCTTGTTGCCCCACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 355 |
| LEAP-Seq percent confirming: | 97.8235 |
| LEAP-Seq n confirming: | 809 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAAGGCATGCAGTCTGTGG |
| Suggested primer 2: | TCCTATCACTGCCTGTGCTG |