Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.197500 |
Chromosome: | chromosome_3 |
Location: | 6017968 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g190950 | TUA1 | Alpha tubulin 1 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CGTGCTCCAGGCAGTAGAGCTCCCAGCAGG |
Internal bar code: | GTGGATGCGGGCGCTCACGGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 777 |
LEAP-Seq percent confirming: | 99.3593 |
LEAP-Seq n confirming: | 3412 |
LEAP-Seq n nonconfirming: | 22 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACCAGGTTGGTCTGGAACT |
Suggested primer 2: | CTCGTGAGAATTGTCTGGCA |