| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.197501 |
| Chromosome: | chromosome 2 |
| Location: | 6170742 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g113652 | (1 of 2) PTHR24320:SF1 - 3-KETODIHYDROSPHINGOSINE REDUCTASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGGGATGCGGTAGCAGGGCCACTCCCT |
| Internal bar code: | ATAGGCCTGGGCCCCGATCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 697 |
| LEAP-Seq percent confirming: | 96.8254 |
| LEAP-Seq n confirming: | 427 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGAAGTGGGCGATGTGGT |
| Suggested primer 2: | GATGAGCTCCACAAAGCCTC |