| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.197519 |
| Chromosome: | chromosome 3 |
| Location: | 3357001 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g166700 | FOR1 | (1 of 3) PTHR23213//PTHR23213:SF214 - FORMIN-RELATED // SUBFAMILY NOT NAMED; Formin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGATGAGCAGCGGGAAGACCAGGAGAAC |
| Internal bar code: | GTTCCCGATGGCGCAAGGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 825 |
| LEAP-Seq percent confirming: | 99.6355 |
| LEAP-Seq n confirming: | 2460 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACACCGTGTAAGCAGCCT |
| Suggested primer 2: | CACCTGACGTGTTGTTGACC |