| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.197605 |
| Chromosome: | chromosome 4 |
| Location: | 3561103 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g228400 | WRK1 | (1 of 1) PF03106 - WRKY DNA -binding domain (WRKY); WRKY family transcription factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACCACGCCCCGACCGCAGCAGCAGTAAA |
| Internal bar code: | GCACATCCCATGGCATTCATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 660 |
| LEAP-Seq percent confirming: | 22.0325 |
| LEAP-Seq n confirming: | 258 |
| LEAP-Seq n nonconfirming: | 913 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCACCATCACAAAGCAA |
| Suggested primer 2: | GTGCGTCCATCCTTACACCT |