| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.197607 |
| Chromosome: | chromosome 1 |
| Location: | 5339643 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGTGCTAAGGCAGTTTGGTGTGGGGCT |
| Internal bar code: | AAAGCGGGGGCCGCAGTCCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 890 |
| LEAP-Seq percent confirming: | 96.6854 |
| LEAP-Seq n confirming: | 6009 |
| LEAP-Seq n nonconfirming: | 206 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTTACTGCACTCGGGTT |
| Suggested primer 2: | CTTGGGCTACACTGGGTGTT |