| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.197609 |
| Chromosome: | chromosome 17 |
| Location: | 5753300 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g739201 | FAP293 | Coiled-Coil Flagellar Associated Protein 293 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTTACACCAGGTCAGCCGGTCAGGTCGG |
| Internal bar code: | GTGCAGACGATGGGAACCTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 995 |
| LEAP-Seq percent confirming: | 99.7722 |
| LEAP-Seq n confirming: | 438 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCATAGTTTGGGGCAGTT |
| Suggested primer 2: | TCTCACGCATTTACACGCTC |