| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.197618 |
| Chromosome: | chromosome 16 |
| Location: | 1771811 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g655050 | NON1 | NO-nitrate 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCCAATCTGGCCCTCGTGCAGCCATCAT |
| Internal bar code: | GAGTTGGACCCGTCAGTTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 895 |
| LEAP-Seq percent confirming: | 75.9542 |
| LEAP-Seq n confirming: | 995 |
| LEAP-Seq n nonconfirming: | 315 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCGCTACATCCCAATAA |
| Suggested primer 2: | CGAGACGAGGACTGATGTGA |