| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.197660 |
| Chromosome: | chromosome 16 |
| Location: | 7707762 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690991 | weak Similarity to Elongation factor 1 | gamma chain; (1 of 1) K03233 - elongation factor 1-gamma (EEF1G) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACGAGTATGTCAGGGCAGAGGCGCTGTC |
| Internal bar code: | CGTACACGATGCACAGCAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 910 |
| LEAP-Seq percent confirming: | 99.3192 |
| LEAP-Seq n confirming: | 2480 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTTGTCTGGACTCTGCTG |
| Suggested primer 2: | CACCTGGCTGACGAGGTATT |