| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.197667 |
| Chromosome: | chromosome 5 |
| Location: | 3450307 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241400 | (1 of 2) 1.16.1.7 - Ferric-chelate reductase (NADH) / NADH:Fe(3+)-EDTA reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTACTCCTCGTAAGTTTAGGTCAAGAGC |
| Internal bar code: | GTAGGATCCTGTCAGTACCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 508 |
| LEAP-Seq percent confirming: | 97.7296 |
| LEAP-Seq n confirming: | 3788 |
| LEAP-Seq n nonconfirming: | 88 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACGAAATAGAGCATCCCC |
| Suggested primer 2: | AGTTCAGCTTTAGGGTGCGA |