| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.197694 |
| Chromosome: | chromosome 7 |
| Location: | 3750722 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338050 | ASA3 | Mitochondrial F1F0 ATP synthase associated protein 3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGTCGACCTAGCCAGTCAGCCAGCTCCA |
| Internal bar code: | TTTGGGGCAGAGTAACTGCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 175 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 425 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATTGTGTGCCCTTTGTTG |
| Suggested primer 2: | TTCAAGGTGTTCAAGGAGGG |