| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.197709 |
| Chromosome: | chromosome 6 |
| Location: | 1014638 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g256000 | (1 of 1) PF15996 - Arginine/serine-rich protein PNISR (PNISR) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGTTCCGGAGCGAAGCAGAACCGATGCA |
| Internal bar code: | GCAAGGCGCGCATCAGCTCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 675 |
| LEAP-Seq percent confirming: | 99.589 |
| LEAP-Seq n confirming: | 727 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACATGAACGGGAGTGAC |
| Suggested primer 2: | TGTTGTTGTTTGCGTTCCAT |