| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.197735 |
| Chromosome: | chromosome 13 |
| Location: | 3484331 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g587700 | SDR33 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAATGGTTGCCCCTGCCTGACCCGCGCAT |
| Internal bar code: | AGACCAGGGCGGTCGGACGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 282 |
| LEAP-Seq percent confirming: | 99.8793 |
| LEAP-Seq n confirming: | 1655 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGATAGGGACAACCTGTGC |
| Suggested primer 2: | GTGTGTGTGTGCGTGTGTGT |