Insertion junction: LMJ.RY0402.197738_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.197738
Chromosome:chromosome 3
Location:3290546
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g166000 (1 of 274) IPR020683 - Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGAAGGGTTTACTCTGCGTACGTGCGGAC
Internal bar code:CCCACAGCCCTGCGCGCGGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:533
LEAP-Seq percent confirming:76.1775
LEAP-Seq n confirming:1116
LEAP-Seq n nonconfirming:349
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TAAGATGTGTGGGCGTGTGT
Suggested primer 2:GTCTGGAGGTGGTGAAGGAG