Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.197758 |
Chromosome: | chromosome_9 |
Location: | 658827 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g403200 | FAP198 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GATACTACACGCCCTACGAGGTCCGTAATA |
Internal bar code: | GGGTCCTAACAGTCGCAGGAAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 664 |
LEAP-Seq percent confirming: | 99.7823 |
LEAP-Seq n confirming: | 1833 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCACTTGATTTTGCTGTCGC |
Suggested primer 2: | CACCAGTGTGTGAGGTCCTG |