Insertion junction: LMJ.RY0402.197764_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.197764
Chromosome:chromosome 8
Location:4206657
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g380350 (1 of 117) IPR002893 - Zinc finger, MYND-type intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAGGCGGCGCTGTGACGGGAAACGCAGGG
Internal bar code:CGAGTCGCTACAGGCTCGATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:370
LEAP-Seq percent confirming:99.6223
LEAP-Seq n confirming:3165
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CATGACCTTACTGCACACCG
Suggested primer 2:GACGTCATTAAGGTCTGCGG