Insertion junction: LMJ.RY0402.197764_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.197764
Chromosome:chromosome 8
Location:4206660
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g380350 (1 of 117) IPR002893 - Zinc finger, MYND-type intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCCCGCGCCAACCTCCAGCTCCCTACTA
Internal bar code:GTGCGGTGTCGAAGTGTGGATT

Confirmation - LEAP-Seq

LEAP-Seq distance:101
LEAP-Seq percent confirming:50.6849
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:36
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CATGACCTTACTGCACACCG
Suggested primer 2:GACGTCATTAAGGTCTGCGG