| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.197800 |
| Chromosome: | chromosome 9 |
| Location: | 4841912 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397549 | (1 of 1) PF06972 - Protein of unknown function (DUF1296) (DUF1296) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGAAATGCACACACAGAGCCTTCTTCTCG |
| Internal bar code: | AACCTCGACGGCAGTGGAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 379 |
| LEAP-Seq percent confirming: | 98.8417 |
| LEAP-Seq n confirming: | 256 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTAGACATCATCGGGGAGC |
| Suggested primer 2: | GCTGTACTGAGAAGGGTCGG |