| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.197813 |
| Chromosome: | chromosome 14 |
| Location: | 1496478 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g618350 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCCAGCACCGCCTGCAGCAGCGGCACG |
| Internal bar code: | GACGTACGCCTGGACATACTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 769 |
| LEAP-Seq percent confirming: | 97.751 |
| LEAP-Seq n confirming: | 1217 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGTAAGCTGCGGTGGAGG |
| Suggested primer 2: | CATGCAGGAGGTAGAGAGGC |