| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.197835 |
| Chromosome: | chromosome 3 |
| Location: | 3281011 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165950 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCCTTCGCGGAGGTGCTGCAGGTGGGTA |
| Internal bar code: | ACTCATCCTCGCTGCGGGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 697 |
| LEAP-Seq percent confirming: | 99.57 |
| LEAP-Seq n confirming: | 3242 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCAGAACCTACCTGAGCG |
| Suggested primer 2: | ACCCACTCCTTCGTATGCAC |