| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.197839 |
| Chromosome: | chromosome_8 |
| Location: | 1017660 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g362050 | FAP49 | Flagellar Associated Protein, membrane-associated | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCCTTATCAAACAAAGGTGGTGGGGTCAT |
| Internal bar code: | ATGCACTCACGGATGCGGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 251 |
| LEAP-Seq percent confirming: | 97.9452 |
| LEAP-Seq n confirming: | 572 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCTTACCACTGTCCCAT |
| Suggested primer 2: | GTTGGGGAGTCAGTCGTTGT |