| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.197841 |
| Chromosome: | chromosome 6 |
| Location: | 3563665 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278117 | CPLD7 | Conserved in the Plant Lineage and Diatoms; (1 of 98) IPR023214 - HAD-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCGGGCTTGTGTATGACTGTATGGTCC |
| Internal bar code: | AGAGTAAACCGGGAAACTAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 852 |
| LEAP-Seq percent confirming: | 99.7234 |
| LEAP-Seq n confirming: | 19111 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTGGCTAAATCTGAGGCT |
| Suggested primer 2: | AATGAGAAAGTGTACGGCGG |