| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.197852 |
| Chromosome: | chromosome 10 |
| Location: | 979869 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424775 | CTP2,PCH1,PAA1 | Copper transporting ATPase and Cu chaperone for CTP1; (1 of 1) PTHR24093:SF124 - COPPER-TRANSPORTING ATPASE PAA1, CHLOROPLASTIC | 3'UTR|intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCGGTATGATCCTACTAGGACGGCACAT |
| Internal bar code: | TATGGGCGGTCGTGGTATCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 990 |
| LEAP-Seq percent confirming: | 99.8021 |
| LEAP-Seq n confirming: | 1513 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTTTAAGGGTTGGGGTC |
| Suggested primer 2: | CCACCTCGTCCTTCATGTTT |