| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.197881 |
| Chromosome: | chromosome 12 |
| Location: | 7455019 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556350 | CFAP300 | (1 of 1) PTHR31339//PTHR31339:SF10 - FAMILY NOT NAMED // PECTIN LYASE-LIKE SUPERFAMILY PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCATGACCTTAGGCGACCACGTTCGCAGT |
| Internal bar code: | GGCTCCCATCGTCCGTCTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 644 |
| LEAP-Seq percent confirming: | 99.8812 |
| LEAP-Seq n confirming: | 841 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCACCTGCATCCATACAC |
| Suggested primer 2: | CGAAGCCAGTGGAGAGCTAC |