Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.197894 |
Chromosome: | chromosome 6 |
Location: | 6868734 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g295450 | HPR1 | (1 of 1) 1.1.1.29 - Glycerate dehydrogenase / Hydroxypyruvate reductase; Hydroxyl pyruvate reductase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATGAGTGCGCAACATGAGAACAGACACG |
Internal bar code: | TGTCGGGAATCCAAGCGTTCAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 637 |
LEAP-Seq percent confirming: | 94.1405 |
LEAP-Seq n confirming: | 18428 |
LEAP-Seq n nonconfirming: | 1147 |
LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGTCTTGTTCTGTGGGTGGG |
Suggested primer 2: | CTACGCAGTGGGCTACAACA |