| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.197960 |
| Chromosome: | chromosome 5 |
| Location: | 1452618 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g244000 | (1 of 1) IPR000253//IPR009836 - Forkhead-associated (FHA) domain // Protein of unknown function DUF1399 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTACTCTACGCGCAGCGCCTGCATGTGC |
| Internal bar code: | GCTTGGTACGCACGCGTACATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 600 |
| LEAP-Seq percent confirming: | 99.7991 |
| LEAP-Seq n confirming: | 2484 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATCTGCTCCAACACTTGC |
| Suggested primer 2: | CTTCTTTGACTACCTGCGCC |