Insertion junction: LMJ.RY0402.197980_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.197980
Chromosome:chromosome 17
Location:1799781
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g709550 (1 of 2) K06709 - jumonji domain-containing protein 2 [EC:1.14.11.-] (JMJD2, JHDM3) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCGTTGCCGAAGGGGTCAAAGGGCGACA
Internal bar code:GAGTCAATGCTGGCGTTTCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:430
LEAP-Seq percent confirming:98.9637
LEAP-Seq n confirming:191
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AACCCGTCAAACTGTATCGC
Suggested primer 2:ATCCCAGTGACGACTAACGG